Detection of bioorthogonal groups by correlative light and electron microscopy allows imaging of degraded bacteria in phagocytes.

Detection of bioorthogonal groups by correlative light and electron microscopy allows imaging of degraded bacteria in phagocytes† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c5sc02905h Click here for additional data file.

Click-electron microscopy for imaging metabolically tagged non-protein biomolecules

EM has long been the main technique for imaging cell structures with nanometer resolution but has lagged behind light microscopy in the crucial ability to make specific molecules stand out. Here we introduce click-EM, a labeling technique for correlative light microscopy and EM imaging of nonprotein biomolecules. In this approach, metabolic labeling substrates containing bioorthogonal functiona...

Correlative cryo-fluorescence light microscopy and cryo-electron tomography of Streptomyces.

Light microscopy and electron microscopy are complementary techniques that in a correlative approach enable identification and targeting of fluorescently labeled structures in situ for three-dimensional imaging at nanometer resolution. Correlative imaging allows electron microscopic images to be positioned in a broader temporal and spatial context. We employed cryo-correlative light and electro...

Correlative Stochastic Optical Reconstruction Microscopy and Electron Microscopy

Correlative fluorescence light microscopy and electron microscopy allows the imaging of spatial distributions of specific biomolecules in the context of cellular ultrastructure. Recent development of super-resolution fluorescence microscopy allows the location of molecules to be determined with nanometer-scale spatial resolution. However, correlative super-resolution fluorescence microscopy and...

CL-SEM for studies on cellular components and infection

In the last years, the combination of different imaging modalities, in particular fluorescence optical light microscopy (FLM) and electron microscopy (EM) techniques, became one of the main challenges. Correlative Light Electron Microscopy (CLEM) methods allow to bridge the resolution gap between ultrastrucural studies and dynamic, functional analysis in cellular systems. Recent advances in lig...